Stepwise Dissociation of Histones from Rat-Liver Chromatin in Alkaline Solutions
نویسندگان
چکیده
منابع مشابه
Metabolic properties of histones from rat liver and thymus gland.
1. The incorporation of (14)C-labelled amino acids into acid-extractable proteins from rat-liver and -thymus nuclei confirmed the existence of a protein component with a higher uptake than that into the major histone components. 2. This rapidly labelled component appeared to contain the thiol groups detectable in the acid extracts. 3. Histone f1 contained 1mol. of serine phosphate/mol. of mol.w...
متن کاملProteins: histones and chromatin.
Patrick Cramer is a professor of Biochemistry at the Ludwig Maximilians University of Munich, Germany, and head of the Gene Center Munich. He is a structural biologist who has contributed to our understanding of the structure and function of RNAP II from yeast and its associated factors. In a recent study, his laboratory described the structure of RNAP II in complex with the initiation factor T...
متن کاملThe phosphorylation of histones in rat liver undergoing malignant transformation.
The incorporation of inorganic phosphate into H1-histones of rat liver was stimulated after the repeated s.c. administration of diethylnitrosamine. This stimulation was observed as early as after the fifth daily injection of the carcinogen and amounts to approximately 3 times the control value on the 60th day of the experiment. The effect was reversible when the application of the carcinogen wa...
متن کاملNatural occurrence of poly(ADP-ribosyl) histones in rat liver.
Poly(ADP-ribose) bound to histones has been isolated from rat liver. When [14C]ribose was administered intraperitoneally to rats at a dosage of 300-750 mug (100-250 muCi)/10o g, approximately 1% of the radioactivity was recovered in the acid (5% CLCCCOOH)-INSOLUBLE MATERIAL OF THE LIVER NUCLEI 2 HR AFTER INJECTION. Of the acid-insoluble radioactivity, 4.5-9% was extractable with 0.25 N HCL. Car...
متن کاملPurification of a phosphoprotein from chromatin of rat liver.
A simple and effective method to purify a phosphoprotein (B2) (Mr 68,000, pI 6.2-8) from phenol-soluble non-histone chromatin proteins of rat liver is described. The purification involved only two steps, CM-cellulose chromatography and preparative SDS/polyacrylamide (10%)-gel electrophoresis. The purified phosphoprotein B2 was shown to be homogeneous by SDS/polyacrylamide-gel electrophoresis. T...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03407.x